什么是ELISA微孔板？一文帶您了解類型、規(guī)格和應用

<p>&nbsp;<font face="宋體" style="font-size: 10.5pt;">　　酶聯(lián)免疫吸附實驗（</font><span style="font-family: Calibri; font-size: 10.5pt;">ELISA</span><font face="宋體" style="font-size: 10.5pt;">）是一種常用的基于板的免疫學領域金標準測定法，用于生命科學和分子生物學實驗檢測和量化復雜混合物中的生物分子。</font></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體">　　在技術上，將抗原或抗體包被在</font>96<font face="宋體">或</font><font face="Calibri">384ELISA</font><font face="宋體">微孔板的表面。然后，將微孔板與抗原特異性抗體一起孵育，這些抗體會與微孔板底部目標抗原結合。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體">　　</font>ELISA<font face="宋體">板是</font><font face="Calibri">ELISA</font><font face="宋體">檢測的核心。它們也被稱為微量滴定板或微孔板，由聚苯乙烯或聚丙烯制成。如今，市場上有各種</font><font face="Calibri">ELISA</font><font face="宋體">板或微孔板可供選擇，以適應不同的實驗室工作流程。因此，要開發(fā)精確、準確和靈敏的生物測定，選擇合適的板至關重要，從而選擇合適的表面來固定目標生物分子。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體">　　在本文中，酶聯(lián)生物將為您介紹下</font>ELISA<font face="宋體">微孔板特點、市場上可買到的微孔板的格式及其多種應用。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <h3><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體">　　</font>Elisa<font face="宋體">板類型</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></h3> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體">　　微孔板有多種類型，每種類型都針對特定的檢測要求和檢測方法量身定制。每個微孔板的表面都對特定生物分子具有高結合親和力。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <ol> <li><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體">　　親和免疫分析板</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li> <li><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體">　　親和免疫測定板是為了增強抗原和抗體的結合而開發(fā)的。它為固定生物分子提供了高親和力的表面，從而提高了測定靈敏度、定量準確度和最佳相互作用。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li> <li><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體">　　這些板用于</font>ELISA<font face="宋體">和免疫測定。它們涂有鎳、鏈霉親和素、抗體和其他配體。它有多種類型，包括：</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li> <li><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體">　　生物素結合板：包括</font>NeutrAvidin<font face="宋體">包被板和鏈霉親和素包被板。這些板通常用于以生物素化分子（如抗體或核酸）作為探針或檢測劑的檢測。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li> <li><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體">　　抗體結合板：抗體結合板的類型包括蛋白</font>A/G/L<font face="宋體">包被板和二抗（</font><font face="Calibri">GAM</font><font face="宋體">、</font><font face="Calibri">GAR</font><font face="宋體">）包被板。它們與各種類型的免疫球蛋白（</font><font face="Calibri">IgG</font><font face="宋體">、</font><font face="Calibri">IgM</font><font face="宋體">和</font><font face="Calibri">IgA</font><font face="宋體">等）和一抗結合，用于高效抗原檢測。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li> <li><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體">　　</font>His<font face="宋體">標簽結合板：鎳和銅涂層板是</font><font face="Calibri">His</font><font face="宋體">標簽結合板的幾種類型。它們廣泛用于涉及組氨酸標記蛋白的測定。</font><font face="Calibri">His</font><font face="宋體">標簽結合板可實現(xiàn)</font><font face="Calibri">His</font><font face="宋體">標簽蛋白的特異性固定和檢測。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li> <li><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體">　　</font>GST<font face="宋體">結合板：包括谷胱甘肽</font><font face="Calibri">(GSH)</font><font face="宋體">涂層板，用于檢測和分析</font><font face="Calibri">GST</font><font face="宋體">標記或谷胱甘肽</font><font face="Calibri">S-</font><font face="宋體">轉移酶</font><font face="Calibri">(GST)</font><font face="宋體">融合蛋白。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li> </ol> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體">　　</font><strong><font face="宋體">吸收免疫分析板</font></strong></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體">　　吸收免疫測定板也稱為結合免疫測定板。這些板是為蛋白質、抗體和其他生物分子的被動吸附（包被）而開發(fā)的。它們被設計為專門針對特點的抗原。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體">　　它也有兩種類型：</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <ol> <li><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體">　　親水性蛋白質結合板：它們旨在吸收親水性的生物分子，例如糖蛋白、抗體、聚糖和其他可溶性蛋白質。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li> <li><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體">　　疏水性脂質結合板：它們被設計用于吸收疏水性的生物分子，例如脂質和脂蛋白。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li> </ol> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體">　　<strong>共價免疫分析板</strong></font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體">　　這些專門設計的微孔板可實現(xiàn)肽、核酸和其他生物分子的共價連接。它們能夠與各種功能基團結合，包括氨基</font>(NH2)<font face="宋體">、巰基</font><font face="Calibri">(SH)</font><font face="宋體">、羧基</font><font face="Calibri">(COOH)</font><font face="宋體">和磷酸基</font><font face="Calibri">(PO4)</font><font face="宋體">，從而實現(xiàn)穩(wěn)定而高效的固定。它們可用于</font><font face="Calibri">ELISA</font><font face="宋體">和其他基于板的檢測技術。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體">　　共價免疫測定板的一個例子是核酸測定板。它們有多種類型，以適應寡核苷酸與各種功能基團或標簽的結合。這些板可以通過共價鍵或親和相互作用實現(xiàn)牢固連接，確保在實驗中準確可靠地分析核酸。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <h3><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體">　　</font>Elisa<font face="宋體">板工作原理</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></h3> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體">　　</font>ELISA<font face="宋體">板是免疫學和診斷實驗室中定期進行</font><font face="Calibri">ELISA</font><font face="宋體">檢測的必備材料。它確保目標抗原的結合并保持目標分析物和抗體之間的化學反應。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體">　　為了開發(fā)有效的免疫測定法，用適當?shù)牡鞍踪|或抗體包被</font>ELISA<font face="宋體">板至關重要。根據(jù)所需的檢測信號選擇合適的微孔板類型對于實現(xiàn)最佳的板包被至關重要。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體">　　檢測微孔板顏色的選擇取決于所檢測的信號。例如，黑色或白色不透明板用于檢測化學發(fā)光和熒光信號，而透明聚苯乙烯平底板用于檢測比色信號。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體">　　確保板上沒有劃痕，以免干擾分析和最終結果。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體">　　包被過程涉及被動吸附，即將蛋白質或抗體添加到板中，并讓其與塑料表面結合。通常，先制備蛋白質或抗體溶液，然后在板上孵育特定時間。孵育后，除去包被溶液，并添加封閉緩沖液以防止非特異性結合。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體">　　需要注意的是，理想包被條件和結合能力可能因所用的特定蛋白質或抗體而異。通常需要通過實驗來確定適當?shù)陌粷舛?。包被板的蛋白質含量略高于所需量可實現(xiàn)更廣泛的檢測范圍，但包被量過大可能導致非特異性結合和高背景信號。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體">　　板子包被后，可立即使用或儲存在</font>4&deg;C<font face="宋體">以備將來使用，具體取決于包被蛋白的穩(wěn)定性。適當?shù)膬Υ鏃l件可確保檢測結果的可靠性和可重復性。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <h3><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體">　　</font>ELISA<font face="宋體">板用途</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></h3> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體">　　</font>ELISA<font face="宋體">聚苯乙烯微孔板在研究、臨床和診斷實驗室及行業(yè)中有著廣泛的應用。它使科研人員能夠量化樣本中目標分子的存在和濃度，例如血液、血清、血漿、尿液或細胞裂解物。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體">　　這些板具有多個孔或隔間，可同時評估多個樣品或測試條件。它們可進行高通量篩選，使研究人員能夠高效處理多個樣品。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體">　　</font>ELISA<font face="宋體">板兼容各種檢測方法，包括比色、熒光和化學發(fā)光技術，為檢測和測量感興趣的抗原提供了靈活性。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體">　　</font><strong>ELISA<font face="宋體">檢測</font></strong></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體">　　</font>ELISA<font face="宋體">是實驗室中最常見和最廣泛使用的基于板的免疫測定技術之一。除了微量離心管和移液器外，該測定還需要</font><font face="Calibri">8</font><font face="宋體">孔條、</font><font face="Calibri">96</font><font face="宋體">孔或</font><font face="Calibri">384</font><font face="宋體">孔</font><font face="Calibri">ELISA</font><font face="宋體">中結合或高結合板。它們允許同時評估多種生物分子。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體">　　對于從事組織培養(yǎng)或基于細胞的分析的研究人員來說，介質結合</font>ELISA<font face="宋體">板為細胞粘附和生長提供了最佳表面。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體">　　孵育和清洗培養(yǎng)板時，必須正確密封培養(yǎng)板，以防止交叉污染并保持樣品的完整性。培養(yǎng)板密封器可以簡化此過程。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體">　　使用</font>ELISA<font face="宋體">板時，請考慮用于產(chǎn)生信號的底物。不同的底物提供各種檢測選項，包括比色和化學發(fā)光信號，讓您可以根據(jù)實驗需要選擇最合適的方法。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體">　　以上就是關于</font>ELISA<font face="宋體">微孔板的介紹。選擇合適的微孔板對</font><font face="Calibri">ELISA</font><font face="宋體">實驗結果至關重要，建議根據(jù)實驗需求，例如結合類型和材質，仔細選擇合適的</font><font face="Calibri">ELISA</font><font face="宋體">微孔板，以確保實驗的成功。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>