elisa底物顯色原理
<p> <font face="宋體" style="font-size: 10.5pt;"> </font><span style="font-family: Calibri; font-size: 10.5pt;">ELISA</span><font face="宋體" style="font-size: 10.5pt;">是一種廣泛應用于生命科學和生物化學實驗室的酶聯(lián)免疫吸附測定實驗，用于定性和定量檢測各種生物分子，例如蛋白質(zhì)、抗體、肽、糖蛋白和激素。</font></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 在</font>ELISA<font face="宋體">實驗中，目標抗原或抗體會被固定在聚苯乙烯微孔板上。然后，加入酶標記的抗體（或抗原，取決于</font><font face="Calibri">ELISA</font><font face="宋體">類型）與目標物質(zhì)進行特異性結(jié)合，并在孵育后形成復合物。未結(jié)合的試劑通過洗滌步驟去除。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 最后，加入特定的底物，酶催化底物反應，產(chǎn)生可檢測的顏色變化或熒光</font>/<font face="宋體">發(fā)光信號。通過測量這些信號，可以定量或定性分析目標物質(zhì)的濃度。</font><font face="Calibri">ELISA</font><font face="宋體">實驗有多種類型，例如雙抗體夾心法、間接法、競爭法等，每種類型都有其特定的操作步驟和應用場景。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> </font>ELISA<font face="宋體">實驗中，在洗滌去除未結(jié)合的酶標記物后，將含有反應結(jié)合物的</font><font face="Calibri">ELISA</font><font face="宋體">板與合適的底物一起孵育。底物的選擇取決于與抗體偶聯(lián)的酶的類型，例如辣根過氧化物酶</font><font face="Calibri">(HRP)</font><font face="宋體">、</font><font face="Calibri">β-</font><font face="宋體">半乳糖苷酶和堿性磷酸酶</font><font face="Calibri">(AP)</font><font face="宋體">。酶催化底物反應，產(chǎn)生可檢測的顏色變化、熒光或發(fā)光信號，從而放大信號并可視化板中發(fā)生的反應。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> </font>ELISA<font face="宋體">實驗中的一些常用底物是過氧化氫、魯米諾和對硝基苯基磷酸鹽</font><font face="Calibri">(pNPP</font><font face="宋體">底物</font><font face="Calibri">)</font><font face="宋體">。在本文中為大家詳細介紹下這些底物在</font><font face="Calibri">ELISA</font><font face="宋體">實驗過程作用方式、它們功能及其在不同醫(yī)學和生物技術實驗室和行業(yè)中的應用。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 在</font>ELISA<font face="宋體">反應體系中，酶標記的抗體（或抗原）與目標抗原（或抗體）結(jié)合后，加入相應的底物。酶會催化底物發(fā)生化學反應，產(chǎn)生可檢測的信號，例如顏色變化、熒光或電化學信號。這些信號的強度與樣本中目標抗原（或抗體）的濃度成正比?？梢允褂梅止夤舛扔?、熒光計或電化學檢測器等儀器測量這些信號，從而定量樣本中目標物質(zhì)的含量。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 不同的底物在與酶發(fā)生氧化反應時會產(chǎn)生不同的顏色。例如，辣根過氧化物酶底物與</font>HRP<font face="宋體">酶反應后會產(chǎn)生以下結(jié)果：</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<ul>
<li><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> </font><font face="Calibri">ABTS</font><font face="宋體">（</font><font face="Calibri">2,2′-</font><font face="宋體">聯(lián)苯二胺</font><font face="Calibri">[3-</font><font face="宋體">乙基苯并噻唑啉</font><font face="Calibri">-6-</font><font face="宋體">磺酸</font><font face="Calibri">]-</font><font face="宋體">二銨鹽）：綠色</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li>
<li><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> </font><font face="Calibri">TMB</font><font face="宋體">（</font><font face="Calibri">3,3′,5,5′-</font><font face="宋體">四甲基聯(lián)苯胺）：橙色</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li>
<li><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> </font><font face="Calibri">OPD</font><font face="宋體">（鄰苯二胺）：藍色</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li>
</ul>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 產(chǎn)生的顏色量或獲得的信號與結(jié)合固定在</font>96<font face="宋體">孔板表面的抗原的一抗的量成正比。</font></span></p>
<p class="MsoNormal"> </p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> <img src="/images/upload/Image/1(84).png" width="500" height="156" alt="" /></span></p>
<p class="MsoNormal"> </p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 為了獲得高質(zhì)量、一致且可靠的蛋白質(zhì)測量結(jié)果，許多科研人員選擇使用</font><a href="/category-1-b0.html">ELISA</a><font face="宋體"><a href="/category-1-b0.html">試劑盒</a>。這些試劑盒提供了進行特定</font><font face="Calibri">ELISA</font><font face="宋體">檢測所需的核心試劑，簡化了操作步驟，縮短了實驗時間。相比于自行配制試劑，使用試劑盒通?？梢詼p少實驗變異性，并提高實驗的重現(xiàn)性。優(yōu)質(zhì)的</font><font face="Calibri">ELISA</font><font face="宋體">試劑盒通常具有較高的靈敏度和批次間一致性。然而，不同品牌的試劑盒性能可能存在差異，研究人員應根據(jù)具體實驗需求選擇合適的試劑盒，并在實驗前仔細閱讀說明書。部分情況下，仍可能需要自行配制或優(yōu)化某些試劑。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal" style="text-align: center;"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> <img src="/images/upload/Image/1 - 副本.jpg" alt="酶聯(lián)生物ELISA試劑盒" width="400" height="300" /></span></p>
<h3><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> </font>ELISA<font face="宋體">底物類型</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></h3>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 根據(jù)所使用檢測方法，</font>ELISA<font face="宋體">底物可分以下幾大類：</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> </font><strong><font face="宋體">化學發(fā)光底物</font></strong></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 報告酶</font>-<font face="宋體">底物反應產(chǎn)生的光被用作化學發(fā)光檢測系統(tǒng)的信號。</font><font face="Calibri">HRP</font><font face="宋體">酶使用的化學發(fā)光底物溶液包括魯米諾。</font><font face="Calibri">AP</font><font face="宋體">酶需要</font><font face="Calibri">1,2-</font><font face="宋體">二氧雜環(huán)丁烷類試劑來產(chǎn)生信號，該信號可被</font><font face="Calibri">X</font><font face="宋體">射線膠片或電荷耦合器件</font><font face="Calibri">(CCD)</font><font face="宋體">相機捕獲。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 為了增加化學發(fā)光信號的強度、持續(xù)時間和靈敏度，檢測過程中也使用增強化學發(fā)光底物</font>(ECL)<font face="宋體">。但應對底物進行稀釋，以防止產(chǎn)生假高強度信號。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> </font><strong><font face="宋體">顯色底物</font></strong></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 這些底物與報告酶反應生成有色的不溶性反應產(chǎn)物，可用分光光度計檢測。該方法中使用的</font>HRP<font face="宋體">底物包括</font><font face="Calibri">2'-</font><font face="宋體">連氮雙（</font><font face="Calibri">3-</font><font face="宋體">乙基苯并噻唑啉</font><font face="Calibri">-6-</font><font face="宋體">磺酸）（</font><font face="Calibri">ABTS</font><font face="宋體">）；</font><font face="Calibri">5-</font><font face="宋體">氨基水楊酸（</font><font face="Calibri">5-AS</font><font face="宋體">）；</font><font face="Calibri">3,3',5,5'-</font><font face="宋體">四甲基聯(lián)苯胺（</font><font face="Calibri">TMB</font><font face="宋體">）；和鄰苯二胺（</font><font face="Calibri">OPD</font><font face="宋體">）。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> </font>AP<font face="宋體">酶的底物是</font><font face="Calibri">5-</font><font face="宋體">溴</font><font face="Calibri">-4-</font><font face="宋體">氯吲哚磷酸鹽（</font><font face="Calibri">BCIP</font><font face="宋體">）和硝基藍四唑（</font><font face="Calibri">NBT</font><font face="宋體">）。通過使用終止液可以增強所產(chǎn)生的比色信號，從而獲得更準確的信號測量結(jié)果。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal" style="text-align: center;"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> <img src="/images/upload/Image/顯色底物.png" alt="顯色底物" width="400" height="459" /></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> </font><strong><font face="宋體">熒光底物</font></strong></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 熒光</font>ELISA<font face="宋體">底物需要熒光計來產(chǎn)生正確的激發(fā)光束，使熒光標簽發(fā)出信號。熒光計通過其激發(fā)和發(fā)射濾光片測量信號。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> </font>ELISA<font face="宋體">檢測中使用的熒光</font><font face="Calibri">AP</font><font face="宋體">底物是</font><font face="Calibri">4-MUP</font><font face="宋體">（</font><font face="Calibri">4-</font><font face="宋體">甲基傘形酮磷酸酯），其在發(fā)射波長為</font><font face="Calibri">440nm</font><font face="宋體">和激發(fā)波長為</font><font face="Calibri">360nm</font><font face="宋體">時產(chǎn)生信號。</font><font face="Calibri">HPPA</font><font face="宋體">（</font><font face="Calibri">3-</font><font face="宋體">對羥基苯基丙酸）和</font><font face="Calibri">HPA</font><font face="宋體">（羥基苯乙酸）底物與過氧化物酶配對。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 為</font>ELISA<font face="宋體">檢測選擇合適的底物取決于靈敏度（檢測下限或上限）、時間要求以及過程中所需</font><font face="Calibri">/</font><font face="宋體">使用的檢測設備類型。</font></span></p>
<p class="MsoNormal" style="text-align: center;"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"><img src="/images/upload/Image/熒光底物.png" alt="熒光底物" width="400" height="402" /></font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<h3><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> </font>Elisa<font face="宋體">底物有何用途？</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></h3>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> </font>ELISA<font face="宋體">底物對于</font><font face="Calibri">ELISA</font><font face="宋體">檢測、擴增和量化信號至關重要。它們與酶發(fā)生反應，產(chǎn)生有色產(chǎn)物或信號，可使用合適的設備進行測量。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> </font>ELISA<font face="宋體">是一種生化檢測方法，用于識別和量化生物分子，例如蛋白質(zhì)、肽、糖蛋白和激素。</font><font face="Calibri">ELISA</font><font face="宋體">板含有抗原抗體（與熒光團或酶結(jié)合）復合物的反應混合物，與合適的底物一起孵育，以進行反應可視化，這是</font><font face="Calibri">ELISA</font><font face="宋體">的最后一步。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 底物與</font>HRP<font face="宋體">或</font><font face="Calibri">AP</font><font face="宋體">酶發(fā)生反應，產(chǎn)生不溶性有色反應產(chǎn)物或信號，通過分光光度計或熒光分光光度計進行測量。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 為</font>ELISA<font face="宋體">選擇正確底物是實驗成功關鍵步驟之一。為您的</font><font face="Calibri">ELISA</font><font face="宋體">實驗選擇合適的底物，最大限度地提高實驗的準確性和可靠性！</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
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